Multi-laboratory evaluation of staphylococcal enterotoxin detection by the RIDASCREEN® SE A, B, C, D, E ELISA kit

Authors

  • Kristina McCallum Colorado Department of Agriculture
  • Robert Phillips USDA FSIS OPHS FERN

DOI:

https://doi.org/10.21423/JRS-V07P001

Keywords:

Staphylococcal enterotoxins, RIDASCREEN, SET, SE

Abstract

The RIDASCREEN® SE A, B, C, D, E kit was evaluated for use as a secondary test for the detection of staphylococcal enterotoxins in meat matrices  Seven state agriculture and public health laboratories participated in the study. The study demonstrated that the kit is able to detect each of the five serotypes in hot dogs, breaded chicken nuggets, bologna, and ready-to-eat barbeque meat. Staphylococcal enterotoxins were detected at a minimum of 1.0 ng/g in all matrices and at levels as low as 0.375 ng/g in some matrices. While cross-reaction between some serotypes is seen, false negative and false positive results were minimally observed. When used along with the BioMerieux VIDAS® SET2 automated immunoassay test, this kit provides a secondary assay to use as confirmation for the presence of staphylococcal enterotoxins in a meat sample.

https://doi.org/10.21423/jrs-v07p001

Author Biographies

  • Kristina McCallum, Colorado Department of Agriculture
    Kristi McCallum is the Deputy Laboratory Manager for the Colorado Department of Agriculture-Biochemistry Laboratory.
  • Robert Phillips, USDA FSIS OPHS FERN
    Robert Phillips, PhD works for USDA-FSIS Office of Public Health Science Food Emergency Response Network.  Dr. Phillips is the Senior Microbiologist.

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Published

2019-01-22

Issue

Vol 7 (2019)

Section

Scientific Articles

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How to Cite

Multi-laboratory evaluation of staphylococcal enterotoxin detection by the RIDASCREEN® SE A, B, C, D, E ELISA kit. (2019). Journal of Regulatory Science, 7, 1-8. https://doi.org/10.21423/JRS-V07P001