Rapid-field-deployable DART-MS screening technique for 87 opioids and drugs of abuse, including fentanyl and fentanyl analogs

Authors

  • Sara Kern US FDA
  • Valerie Toomey US Food and Drug Administration
  • Lisa Lorenz US Food and Drug Administration

Keywords:

ambient ionization, mass spectrometry, field deployable instrumentation, opioids

Abstract

Fentanyl and fentanyl analogs are playing an increasing role in the opioid health crisis that is sweeping America. These synthetic opioids are much more potent and lethal than morphine or heroin and must be treated with extreme caution. Many of these substances are entering the country through International Mail Facilities (IMFs) and Express Courier Hubs (ECHs) in the form of counterfeit prescription drugs and bulk powders, resulting in a critical need for field-deployable techniques that can safely provide rapid screening to prevent these products from reaching the public.  Here we describe a direct analysis in real time thermal desorption mass spectrometer (DART-MS) with database searching capabilities that allows quick detection of numerous target compounds. Minimum detectable levels for 87 opioids and drugs of abuse have been determined. This technique was applied to eight samples sent to the Food and Drug Administration’s (FDA) Forensic Chemistry Center for analysis and the results are compared to those collected using gas chromatography with mass spectral detection (GC-MS) and liquid chromatography with mass spectral detection (LC-MS).

References

Ahmad F.B., Rossen L.M., Sutton P. Provisional drug overdose death counts. Products - Vital Statistics Rapid Release - Provisional Drug Overdose Data (cdc.gov). National Center for Health Statistics. 2021.

Cooman T., Ott C.E., Dalzell K.A., Burns A., Sisco E., Arroyo L.E. (2021). Screening of seized drugs utilizing portable Raman spectroscopy and Direct Analysis in Real Time-Mass Spectrometry (DART-MS). Forensic Chemistry. https://doi.org/10.1016/j.forc.2021.100352

de O. Penido C.A.F., Pacheco, M.T.T., Lednev I.K., Silveira L., (2016) Raman spectroscopy in forensic analysis: identification of cocaine and other illegal drugs of abuse, Journal of Raman Spectroscopy, 47 28–38. http://dx.doi.org/10.1002/jrs.4864

Drug Enforcement Administration, U.S. Department of Justice, DEA-DCT-DIB-021-16, Counterfeit Prescription Pills Containing Fentanyls: A Global Threat 2. (2016).

FDA’s Budget: Advancing the Goal of Ending the Opioid Crisis. FDA’s Budget: Advancing the Goal of Ending the Opioid Crisis | FDA, (accessed August 27, 2021).

Lanzarotta A., Kern S., Batson J., Falconer T.M., Fulcher M., Gaston K.W., Kimani M.M., Lorenz L., Morales-Garcia F., Ranieri N., Skelton D., Thatcher M.D., Toomey V.M., Voelker S., Witkowski M.R. (2021) Evaluation of “Toolkit” consisting of handheld and portable analytical devices for detecting active pharmaceutical ingredients in drug products collected during a simultaneous nation-wide mail blitz. Journal of Pharmaceutical and Biomedical Analysis, 203 114183. https://doi.org/10.1016/j.forc.2020.100294

Lanzarotta A., Witkowski M., Batson J. (2020) Identification of Opioids and Related Substances using Handheld Raman Spectrometers. Forensic Science, 65(2) 421-427, http://dx.doi: 10.1111/1556-4029.14217

Mainali D., Seelenbinder J. (2016) Automated fast screening method for cocaine identification in seized drug samples using a portable Fourier transform infrared (FT-IR) instrument. Applied Spectroscopy, 70 916–22. https://doi: 10.1177/0003702816638305.

National Drug Threat Assessment, Drug Enforcement Agency, (2018). 2018 National Drug Threat Assessment (NDTA) (dea.gov)

] Sisco E. and Forbes T.P. (2021) Forensic applications of DART-MS: A review of recent literature. Forensic Chemistry, 22 100294. https://doi.org/10.1016/j.forc.2020.100294

Sisco E., Forbes T.P., Staymates M.E., Gillen G. (2016) Rapid analysis of trace drugs and metabolites using a thermal desorption DART-MS configuration. Analytical Methods, 8 6494-6499. http://dx.doi.org/10.1039/C6AY01851C.

Sisco E., Robinson E., Burns A., Mead R. (2019) What’s in the bag? Analysis of exterior drug packaging by TD-DART-MS to predict the contents. Forensic Science International, 304 1-7. http://dx.doi.org/10.1016/j.forsciint.2019.109939

Sisco E., Verkouteren J., Staymates J., Lawrence J. (2017) Rapid detection of fentanyl, fentanyl analogues, and opioids for an on-site or laboratory based drug seizure screening using thermal desorption DART-MS and ion mobility spectrometry. Forensic Chemistry 4 108-115. http://dx.doi.org/10.1016/j.forc.2017.04.001.

Sorak D., Herberholz L., Iwascek S., Altinpinar S., Pfeifer F., Siesler H.W. (2012) New developments and applications of handheld Raman, mid-infrared, and near-infrared spectrometers. Applied Spectroscopy Reviews, 47 83–115. https://doi.org/10.1080/05704928.2011.625748

Wide-ranging online data for epidemiologic research (WONDER). Atlanta, GA: CDC, National Center for Health Statistics; 2020. Available at http://wonder.cdc.gov.

Zaknoun, H., Binette M.-J., Tam M. (2019) Analyzing fentanyl and fentanyl analogues by ion mobility spectrometry. International Journal of Ion Mobility Spectrometry, 22 1–10. http://dx.doi.org/10.1007/s12127-019-00244-0.

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Published

2022-05-22 — Updated on 2022-05-26

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